The Use of Proteomics and Bioinformatics to Develop a Serum Based Diagnostic Test for Feline Lymphoma
Tariq Shah
Institution: PetScreen Inc
Address: Missouri University Business Incubator,
1601 S. Providence Rd, Columbia,
Missouri, 65211, USA
Abstract:
Feline lymphoma commonly occurs without peripheral lymphadenopathy, providing a diagnostic challenge for general practitioners. Development of a serologic test to detect markers associated with lymphoma would be useful for both diagnosis and monitoring the response to therapy. Mass spectrometry based proteomic technologies are currently at the forefront for the discovery of disease indicative biomarkers. This protocol being developed could offer huge potential within the field of human diagnostics.
Serum was collected from cats with lymphoma and other diseases, and from healthy cats. Two different fractionation methodologies were used prior to mass spectrometric analysis - Ion exchange chromatography and Ceres-Nano technology using Nanotraps® (Hydrogel nanoparticles consisting of a N-isopropylacrylamide core, conjugated to an affinity based bait, and surrounded by a NIPAm shell).
Several significantly different peaks between the 2 groups were identified: 3 from ion exchange chromatography and 7 from the Nanotrap technology. Preliminary Classification And Regression Tree analysis of the data produced a decision tree capable of differentiating between the lymphoma and control groups giving a sensitivity and specificity of 75% and 85% respectively.
This preliminary work supports the ongoing program to recruit further samples in order to develop an accurate feline lymphoma blood test.
